Description
Omega Biotek Mag-Bind® SeqDTR
Removal of unincorporated terminators from sequencing reactions using magnetic beads
The Mag-Bind® SeqDTR is designed for efficient and reliable removal of unincorporated dye terminators from sequencing reactions. The system combines Omega Bio-tek’s proprietary chemistry with the reversible nucleic acid-binding properties of paramagnetic beads to eliminate excess nucleotides, primers, salts, and unincorporated dye terminators. This kit is designed for both manual and fully automated purification of sequencing products, and the protocol has been successfully automated on liquid handlers such as Beckman Coulter’s Biomek® FX/NX, and the Hamilton Microlab® STAR/STARlet. With the high recovery rate from the Mag-Bind® SeqDTR Kit, typically only 1/16 or 1/32 of the ABI Big Dye reagents are needed for sufficient signal strength when compared to sephadex-based clean ups.
- No protocol change against major competitor
- Read lengths averaging over 800 bps (Min Phred 20)
- Manual or adjustable to automated liquid handlers
- Significant cost savings compared to sephadex-based clean ups
- 96- or 384-well formats
| FEATURES | SPECIFICATIONS |
|---|---|
| Downstream application | Sanger Sequencing, Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting |
| Elution volume | 40 µL or above |
| Starting material | ABI Big Dye Chemistry Cycle Sequencing Reactions |
| Starting amount | 5 – 20 µL |
| DNA recovery | Sequencing reads start at 40 nt |
| Processing mode | Automated; Manual |
| Throughput | 96-384 samples per run |
| DNA binding technology | Magnetic beads |
| Binding capacity | Scalable |
| Storage | 2°C – 8°C |
