Description
DIM.CE: HDV IgM – ELISA
Enzyme ImmunoAssay (ELISA) for the determination of IgM class antibodies to Hepatitis Delta Virus or HDV in human plasma and sera with the “capture” system. The kit is intended for the classification of the viral infective agent and the follow-up of HDV infected patients. For “in vitro” diagnostic use only. Microplates are coated with a monoclonal anti-hIgM antibody that in the 1st incubation “captures” specifically this class of antibodies. After washing out all the other components of the sample, in the 2nd incubation bound anti HDV IgM are detected by the addition of recombinant HDV antigen immunocomplexed with a specific antibody, labeled with peroxidase (HRP). After washing, the enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of IgM antibodies present in the sample.
DAG.CE: HDV Ag – ELISA
Third generation Enzyme ImmunoAssay (ELISA) for the determination of Hepatitis Delta Virus or HDV in human plasma and sera. The kit is intended for the follow-up of HDV infected patients. For “in vitro” diagnostic use only. HDV Ag, if present in the sample, is captured by a specific monoclonal antibody, in the 1st incubation. A detergent is added to the sample in order to dissolve the specific antigen from HDV particles. In the 2nd incubation, after washing, a tracer, composed of a second anti HDV Ag antibody, labeled with peroxidase (HRP), is added to the microplate and binds to the captured HDV Ag. The concentration of the bound enzyme on the solid phase is proportional to the amount of HDV Ag in the sample and its activity is detected by adding the chromogen/substrate in the 3rd incubation. The presence of HDV Ag in the sample is determined by means of a cut-off value that allows for the semi quantitative detection of the antigen.
DAB.CE: HDV Ab – ELISA
Competitive Enzyme ImmunoAssay (ELISA) for the determination of antibodies to Hepatitis Delta Virus or HDV in human plasma and sera with a “two-steps” methodology. The kit is used for the follow-up of patients infected by HDV. For “in vitro” diagnostic use only. Anti-HDV antibodies, if present in the sample, compete with a virus-specific polyclonal IgG, labeled with peroxidase (HRP), for a fixed amount of rec-HDV coated on the microplate. The test is carried out with a two steps incubation competitive system. First the sample is added to the plate and specific anti HDV antibodies bind to the adsorbed antigen. After washing, an enzyme conjugated polyclonal antibody to HDV is added and binds to the free portion of the antigen coated. After washing a chromogen/substrate mixture is dispensed. The concentration of the bound enzyme on the solid phase becomes inversely proportional to the amount of anti-HDV antibodies in the sample and its activity is detected by the added chromogen/substrate. The concentration of HDV-specific antibodies in the sample is determined by means of a cut-off value that allows for the semi quantitative detection of anti-HDV antibodies.
