Autoimmunity – ELISA

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Description

For in vitro diagnostic use only.

SSA52.CE: IgG anti SSA 52 KD – ELISA

The device is an Enzyme ImmunoAssay (ELISA) intended to be used for the quantitative determination of IgG autoantibodies against SSA-52KD autoantigens in human plasma and sera.

Microplates are coated with a preparation of Recombinant SSA 52KDa antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti-SSA52 IgG are captured, if present, by the solid phase. After washing out all the other components of the sample, in the 2nd incubation the bound anti-SSA52 IgG are detected by the addition of anti hIgG antibody, labeled with peroxidase (HRP). The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-SSA52 IgG antibodies present in the sample. IgG in the sample may be quantitated by means of a standard curve calibrated in arbitrary units per milliliter (arbU/ml) as no international standard is available.

SSA60.CE: IgG anti SSA 60 KD – ELISA

The device  is an Enzyme ImmunoAssay (ELISA) intended to be used for the quantitative determination of IgG autoantibodies against SSA-60KD autoantigens in human plasma and sera.

Microplates are coated with a preparation of recombinant SSA 60KDa antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti-SSA60 IgG are captured, if present, by the solid phase. After washing out all the other components of the sample, in the 2nd incubation bound anti-SSA60 are detected by the addition of anti hIgG antibody, labeled with peroxidase (HRP). The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-SSA60 IgG antibodies present in the sample. IgG in the sample may be quantitated by means of a standard curve calibrated in arbitrary units per milliliter (arbU/ml) as no international standard is available.

SSB.CE: IgG anti SSB – ELISA

The device  is an Enzyme ImmunoAssay (ELISA) intended to be used for the quantitative determination of IgG autoantibodies against SSB autoantigens in human plasma and sera.

Microplates are coated with a preparation of recombinant SSB antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti-SSB IgG are captured, if present, by the solid phase. After washing out all the other components of the sample, in the 2nd incubation bound anti-SSB are detected by the addition of anti hIgG antibody, labeled with peroxidase (HRP). The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-SSB IgG antibodies present in the sample. IgG in the sample may be quantitated by means of a standard curve calibrated in arbitrary units per milliliter (arbU/ml) as no international standard is available.

RNP.CE: IgG anti U1 – sn RNP68 – ELISA

Enzyme ImmunoAssay (ELISA) for the quantitative determination of IgG autoantibodies to RNP-68KDa in human plasma and sera.

Microplates are coated with a preparation of recombinant U1-snRNP 68 antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti U1-snRNP 68 IgG are captured, if present, by the solid phase. After washing out all the other components of the sample, in the 2nd incubation bound anti U1-snRNP 68 are detected by the addition of anti hIgG antibody, labeled with peroxidase (HRP). The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti U1-snRNP 68 IgG antibodies present in the sample. IgG in the sample may be quantitated by means of a standard curve calibrated in arbitrary units per milliliter (arbU/ml) as no international standard is available.

SM.CE: IgG anti Sm – ELISA

The device  is an Enzyme ImmunoAssay (ELISA) intended to be used for the quantitative determination of IgG autoantibodies against Sm autoantigens in human plasma and sera.

Microplates are coated with a preparation of purified Sm antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti-Sm IgG are captured, if present, by the solid phase. After washing out all the other components of the sample, in the 2nd incubation bound anti-Sm are detected by the addition of anti hIgG antibody, labeled with peroxidase (HRP). The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-Sm IgG antibodies present in the sample. IgG in the sample may be quantitated by means of a standard curve calibrated in arbitrary units per milliliter (arbU/ml) as no international standard is available.

CENPB.CE: IgG anti Centromere B – ELISA

The device CENPB.CE is an Enzyme ImmunoAssay (ELISA) intended to be used for the quantitative determination of IgG autoantibodies against Centromere B autoantigens in human plasma and sera.

Microplates are coated with a preparation of recombinant Centromere B antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti-CENPB IgG are captured, if present, by the solid phase. After washing out all the other components of the sample, in the 2nd incubation bound anti-CENPB are detected by the addition of anti hIgG antibody, labelled with peroxidase (HRP). The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-CENPB IgG antibodies present in the sample. IgG in the sample may be quantitated by means of a standard curve calibrated in arbitrary units per milliliter (arbU/ml) as no international standard is available.

JO1.CE: IgG anti Jo-1 – ELISA

The device  is an Enzyme ImmunoAssay (ELISA) intended to be used for the quantitative determination of IgG autoantibodies against Jo-1 autoantigens in human plasma and sera.

Microplates are coated with a preparation of recombinant Jo-1 antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti Jo-1 IgG are captured, if present, by the solid phase. After washing out all the other components of the sample, in the 2nd incubation bound anti Jo-1 IgG are detected by the addition of anti hIgG antibody, labeled with peroxidase (HRP). The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti Jo-1 IgG antibodies present in the sample. IgG in the sample may be quantitated by means of a standard curve calibrated in arbitrary units per milliliter (arbU/ml) as no international standard is available.

SCL70.CE: IgG anti Scl-70 – ELISA

The device  is an Enzyme ImmunoAssay (ELISA) intended to be used for the quantitative determination of IgG autoantibodies against Scl-70 autoantigens in human plasma and sera.

Microplates are coated with a preparation of recombinant Scl-70 antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti-SCL70 IgG are captured, if present, by the solid phase. After washing out all the other components of the sample, in the 2nd incubation bound anti-SCL70 are detected by the addition of anti hIgG antibody, labeled with peroxidase (HRP). The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-SCL70 IgG antibodies present in the sample. IgG in the sample may be quantitated by means of a standard curve calibrated in arbitrary units per milliliter (arbU/ml) as no international standard is available.

ANAS.CE: ANA Screening IgG – ELISA

Enzyme ImmunoAssay (ELISA) for the qualitative determination of IgG auto-antibodies to dsDNA, histones, SSA, SSB, Sm, Sm/RNP, Scl-70, Jo-1, centromere and other antigens extracted from the HEp-2 nucleus, in human plasma and sera.

ANA8PRO.CE: ANA 8 parameters profile – ELISA

Enzyme ImmunoAssay (ELISA) for the qualitative determination of IgG auto-antibodies to Ro/SSA 60KDa, Ro/SSA 52KDa, La/SSB, RNP-68, Sm, Scl-70, Jo-1, CENPB, in human plasma and sera.

ENAS.CE: ENA Screening IgG – ELISA

Enzyme ImmunoAssay (ELISA) for the qualitative determination of IgG auto-antibodies to SSA, SSB, Sm, RNP68, Scl-70, Jo-1, in human plasma and sera.

ENA6PRO.CE: ENA 6 parameters profile – ELISA

The device is an Enzyme ImmunoAssay (ELISA) intended to be used for the qualitative determination of IgG autoantibodies against Ro/SSA, La/SSB, RNP-68, Sm, Scl-70, Jo-1 in human plasma and sera.

Additional information

Option

IgG anti SSA 52 KD, IgG anti SSA 60 KD, IgG anti SSB, IgG anti U1 – sn RNP68, IgG anti Sm, IgG anti Centromere B, IgG anti Jo-1, IgG anti Scl-70, ANA Screening IgG, ANA 8 parameters profile, ENA Screening IgG, ENA 6 parameters profile